Bước đầu đánh giá mức độ an toàn vệ sinh thực phẩm trên tôm sú nuôi thâm canh theo hệ thống đa chu kì - Đa áo tại Hải Phòng - Nguyễn thị Biên Thúy

Nội dung text: Bước đầu đánh giá mức độ an toàn vệ sinh thực phẩm trên tôm sú nuôi thâm canh theo hệ thống đa chu kì - Đa áo tại Hải Phòng - Nguyễn thị Biên Thúy

1. KHOA HOC CbNG NGHfi aVUC OAU DAIMH GIA IVlUC DD AIM TDAIM VE 5IIMH THUC PHAIVI TREIM TDIVI SU [Penaeus manadati^ iMUDI THAIVI CAIMH THED HE THDIMG DA CHU KY- DA AD TAI HAI PHOIMG Le Ngpc QudnS Bin Quang Tl^, Nguyin Thj Bien Thiiy^, Hodng Thj Yln^ Truong Thi Thdnh Vinh^ T6MTAT Nuoi tom su dam bao an toan ve sinh thuc pham hien nay rat can thiet, trong do cac giai phap ngay tir khau nuoi trong la rat quan trong. Nghien ciiu mot sd chi tieu vi sinh vat tren tdm su {Penaeus monodon) nuoi thdm canh theo hd thdng da chu ky - da ao (nuoi tom theo cac giai doan khac nhau. mdi cdp ao tuong img vdi mot giai doan smh trucmg cua torn va mdi cap ao dupc nuoi lap lai nhieu lan trong mpt nam) da dupc tien hanh. Bdng phuang phap dinh lupng (theo phuong phap MNP, S. D. Millar va G. N. Frerchs ), nhan dang trdn mdi trudng nudi cay dac trung, thir mpt so phan iing sinh hoa cho thay: Vi khuan hiiu khi tdng sd. Fecal coliform, E .coli, Vibrio spp: Staphylococcus spp. Salmonella diu nim trong gidi ban cho phep ciia tieu chuan Viet Nam va the gidi hoac neu co thi anh huong khdng dang ke de'n ngudi tidu dimg sir dung san pham cd loai vi khuan tren. Tuy nhien, su cd mat cua chirng trdn ddi tupng nudi cd thi la nguyen nhdn gdy benh cho tdm neu nhu cac yeu td mdi trudng ao nuoi khdng thuan lpi. Mat dp te bao cua vi khuan hie'u khi tong sd. Fecal coliform, Vibrio spp b cip 1 Idn nhdt va co su sai khac c6 y nghia thdng kd voi mo hinh 2 cdp va 3 cap, con giCra md hinh 2 cap va 3 cap khdng cd su sai khac co y nghia thdng kd vdi a = 0,05. Mat dp ii bao E. coli, Staphylococcus spp d md hinh 1 cap Idn nhat, tuy nhien ph&n tich thdng kd cho thay khdng c6 su sai khac gitra 3 md hinh. Phan tich du lupng thuoc, khang sinh sau khi tdm sii dat kich ca thuong pham cho thay khdng phat hien dupc cac mau nhidm cloramphenicol, nitrofuran, tetracyclin, sulphonamid, flofenicol, fluoroquinolon, malachite green, dipterex, aflatoxin. Tirkhda.-^ toan v$sinh thucpham, cho phep, gidi h^, tiduchuan, saikhac. L OAT VAN D£ *" (Penaeus monodori) nudi thdm canh theo h| thdng , , . da chu ky- da ao (thude dl tdi cap Nhd nudc, md sd Tdm su Id md,' t tron. g nhung thu, e phdm ,<dup; e KC-07.11/06-10vn t^n ^^ ic\tiAr.\) da A- dup A c 'til An han' u-h vou l mu'• c tiiu:*A nguoi tiiu dung a nhidu nuoc ua chudng, no la san pham chu luc dl xudt khau cua Viet Nam. Tuy nhidn, ^^nh gid miic dd nhilm mdt sd vi sinh vdt, du ti-ong qud trinh nudi, cd till bi nhidm mdt sd lodi vi "^^^^ hod chat, tiiudc khdng sinh dnh hudng din an khuan tir mdi tmdng tiiidn nhien, mdi truong nudi ^odn vd sinh tiiuc pham tren tdm sii tiiuong pham; trdng, luu giir, ehi biln din bao qudn vd cung ung So sdnh mirc dp nhilm trong edc cdp nudi khde khdng thich hpp. Ddc biet. cd till nhilm cdc loai phay nhau cua 3 md hinh. khuan td, Sahmonelaa, Shigelle, E.coli gdy ngd ddc |_ y/jy ypj yj| p||||0|\|G piup NGHBV CUU vd dan din tii vong. Chinh vi vdy, hiin nay van dl . .^ * , , , , , , , , , , ' U-' .» A ' A^^M. u- A'' •' 1. D;a diem, thdi gian nghiin cmi vd ky thu^t chat lupng san pham dang dupc ddt len hang dau ^ ., nhilu qudc gia. trd thdnh rdo can quan trpng ddi vdi cdc mdt hdng xudt khau vdo cdc tiii tmdng Idn nhu • Bja diln nghiin cim: tai khu nudi tdm sii tiidm My, EU csr^ ciia dl tdi cap Nhd nudc, md sd KC-07.11/06-10. .1. , ,^^, ,. -• *i ^- A- \. •• - J- Tram Nghiin ciru Nudi trdng Thiiy san nudc lo, Vidn Xuat phat tu nhung van dl tren, danh gia muc dp ,,•, , ,,, , ^, , , , . Thanh, qudn Duong Kinh, TP. Hai Phdng. vat vd du ,lupn . ,g ^ho, d chat , thude khdn-4. -g' sinu'*;h -ti-ln • td•m u Nghien cuu Nuoi trong Thuy san 1. phuang Hai an toan vd sinh thirc pham qua mpt so cm tieu VI sinh _^ - ,Thd i . gia_^n thui^c - hiet nT Dti rTI-DU thdng- 4 din thdng ' Trung tam Khuyen ndng Quoc gia 11/2009. So dd thi nghi|m h| thdng nudi da chu ky- ^ Vi?n Nghien ciru NTTS I da ao bd tri nhu sau: Trirdng D^i hpc Vinh NONG NGHlfP VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011 '^
2. KHOA HOC CbNG NGHfi + Md hinh 1 cap bd tri 2 ao: Ao B7, dien tich 800 - Ky thudt nudi: Ap dung quy trinh nudi tdm it nr; ao B9, diln tich 800 ml thay nudc. -I- Md hinh 2 cap bd tri 3 ao: Ao A5, diln tich 400 + Tieu chuan gidng tdm sii P15 khdng nhilm m^; A ao B6 di|n tich 800 m^; ao B8 diln tich 800 ml benh vi nit ddm trdng (WSSV), benh dau vdng + Md hinh 3 cap bd tri 3 ao: Ao Al, diln tich 400 (YHD), benh MBV (nlu ed nhilm MBV, ty le < 20%) ml ao A2 dien tich 800 ml ao A3 dien tich 1.600 ml va benh IHHNV. + He thdng ao Idng, ao xir ly, ao lpc, mdi ao didn + Sir dung chi pham sinh hpc trong sudt qiia tich 800 ml trinh nudi: gdy mdu nudc; tao siUe, edc ehi pham vi sinh vdt: Aquapond-100, Pond-Clear tdng cudng vi khuan hiru ich phdt trien de ire ehi ede vi khuan gdy benh trong sudt qua trinh nudi. + Thirc dn sir dung trong nghiin ciiu: Thirc dn tiieo tieu chuan 28 TCN 102: 2004, khdng ed khang sinh va hda chat cam sir dung trong nudi trdng thiiy san (tiieo Quyit dinh sd 07/2005/QD-BTS, ky ngay 24/02/2005). Chpn tiiirc dn Concord. + Mat dp tha, thdi gian nudi cua mdi ao eiia he thdng nudi da chu ky - da ao dupc trinh bdy trong Hinh 1. So dd md hinh 1, 2, 3 cdp bd tdi 2 vd 3 ao dupc bang 1. xdy dung tai diim nghien cuu B ang 1.1Vid t do thd vd thdi gian nudi ciia mdi md hinh t^ dilra nghidn ciiu Md Ao Dien tich Dd sdu Mat dp Thdi Thdi gian Thdi gian Ao hinh cap (cm) (con/m^ gian tha tiiu nudi (ngdy) 1 B7 800 120,41 ± 3,02 35 7/4 9/8 125 1 cap 1 B9 800 120,41 ± 3,02 35 24/6 6/10 124 Vui 1 A5 400 122,0 ± 6,03 150 7/4 20/5 43 2 B6 800 125,8± 2,44 31 20/5 10/8 81 2 B8 800 125,a± 2,57 31 20/5 11/8 82 2 cap Vu2 1 A5 400 122,0 ± 6,03 150 10/7 20/8 41 2 B6 800 125,8± 2,44 32 20/8 8/11 80 2 B8 800 125,at 2,57 32 20/8 8/11 80 Vui 1 Al 400 120,3 ± 6,35 150 14/4 26/5 42 2 A2 800 125,0± 2,76 67,5 26/5 8/7 43 3 A3 1.600 124.2± 3.19 28 8/7 22/8 43 3 cap Vu2 1 Al 400 120,3 ± 6.35 175 4/6 14/7 40 2 A2 800 125,0± 2,76 72 14/8 24/9 41 3 A3 1.600 124,2± 3,19 32 24/9 5/11 42 2. Npi dung nghidn aiu dipterex/trichlorfon, nhdm teti-acyeline, nhdm - Dinh lupng vi khuan hieu khi tdng sd, vi khuan sulfonamide, florfenicol, nhdm fluoroquinolones liln gay benh (E. coli, Salmonella spp. Staphylococcus quan den an toan vl sinh thtrc pham. spp. Vibrio spp) trin tdm su thuong pham. 3. Phuong phdp nghiin ciiu - Phdn tich du lupng hod chat, thudc khdng sinh: Chloramphenicol, nhdm nitrofurans, xanh malachite. - Phuong phdp thu va bao quan mau 76 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011
3. KHOA HOC CbNG NGHfi Sir dung vd ddt d cde diim khde nhau trong ao, - Phuang phap phdn tich du lupng hod chat, cho tdm vao tiii ni Idng cd bom oxy vd van chuyin ve thudc khang sinh lien quan den an toan vd sinh thuc phdng thi nghiim, giu- trong tii lanh vd phan tich pham bao gdm: Phuang phdp ndi bd/FDA LIB#4306- trong vdng 24 gid kl tir luc lay mdu. 2003 phan tich du luang Cloramphenicol; phuong - Sd lupng mau thu phap ndi bd/FDA, Aprrill, 2004 phan tich du luong Tdm sil Penaeus monodon thuang pham dupc nitrofuran; phuong phdp AOAC 995-09-2002 phdn thu tir cdc ao nudi cua h| thdng nudi 1, 2 vd 3 cap. tich du lupng tetracyclin; phuang phdp ndi bd/FST- Mdi cdp ao thu mdu 1 lan (15 con /I Ian), thdi gian WIOl-11 phdn tich du lupng sulphonamit; phuong thu tmdc khi thu hoach tdm, hodc chuyin ao 10-15 phdp ndi bd/FDA LIB#4333 phdn tich du lupng ngdy. flofenicol va malachit green; phuang phap ndi bd/FDA LIB#4298-2003 phan tich du luong - Dinh lugng Fecal coliform tren tdm sti fluoroquinolone; phuong phdp AOAC 983.21-2205 Theo phuong phdp MNP (Most Probable phdn tich du lupng dipterex; phuang phdp AOAC Number technique): Tdng sd Fecal coliform dupc 49.2.05 phdn tich du lupng aflatoxin. dpc d Id u 44.5"C/ 24gid. Quan sat sir hinh thdnh bpt khi trong dng phao (kit qua duong tinh); diing bang 4. Phuang phdp xu Iy, phdn tich sd lilu MPN- 3 dng dl tinh lupng Fecal coliform cd xac suat Sd lieu dupe tdng hop phdn tich, danh gid theo Idn nhat trong mau. phuang phap thdng ke sinh hpc vdi su hd tro ciia - Dmh lupng vi khuan hieu khi tdng sd. E. coli. phan mem Excel. Phan tich phuang sai mdt nhdn td Salmonella, Staphylococcus, Vibrio choleraeva V. dupc diing de so sanh mat dp tl bdo cdc vi sinh vdt parahaemolyticus trdn tdm su thuang pham giura 3 md hinh nudi. Xdc dinh mirc dd nhilm khuan theo phuang 1. KET QUA VA THAO LUAN phdp dinh lupng vi khuan (dim sd khuan lac true 1. Kit qud khdo sdt miic dd nhilm vi khudn hiiu tiip trdn mdi trudng nudi cay) ciia S. D. Millar vd G. khi tdng sd N. Frerchs (Stiriing. 1984,1993). Kit qua (bang 2) cho thay, khdi lupng vd chilu Trdn mdi trudng dinh dudng Id thach, ii d 28 - ddi tdm nudi d 3 md hinh khdng cd sir sai khde; 100% 30"C trong 18 - 24h, dim sd khuan lae ciia vi khuan edc mau tdm kilm tra d ca 3 md hinh nudi trdn diu hieu khi tdng sd; trin mdi tmdng thach Macconkey. cd mdt vi khuan vdi mirc tmng binh dao ddng tir li d 28 - 30"C trong 18 - 24 gid, dim sd khuan lac cd 3,4.10^ den 1,2.10^ cftj/g. mdu dd ednh sen, xung quanh cd viing md suong ciia E.coli; tren mdi trudng thach-bistuth sunphate (S. S. Theo quy dinh hien hanh d Viet Nam (ca tidu agar), ii d 35"C/40 - 48 gid, dim sd khuan lac mau chuan ngdnh va tieu chuan Viet Nam) thi chi tieu nay den dng dnh kim loai (hodc khuan lae nhat mdu) ciia can d mirc 5.10^ cfii/g (Thdng tu sd 29/2010/TT- Salmonella; trin mdi trudng Chapmen, u d 28 - BNNPTNT [1]; Quyit dinh sd 46/2007/QD-BYT eua 30"C/24 - 48 gid, dim sd khuan lac mau vang cua Bd Y tl [2]). Tuy nhidn d mdt sd thj tmdng tieu thu Staphylococcus; trin mdi trudng TCBS, ii d 30"C/18- Idn nhu Nhdt Ban [5], ehi sd nay ed thi d mire cao 24 gid, dim sd khuan lae mau vdng (dudng kinh 2-3 han (<3,0 .10«CFU/g).Th| tiardng EU ddi hdi nghiem mm) ciia V. cholerae vd mdu xanh (dudng kinh 3-5 ngdt ban, mirc chap nhdn dupc phai <10^ CFU/g. mm) cua Vibrio parahaemolyticus; Nhu vdy, tdm sii nudi trong ea 3 md hinh trong nghidn cim ndy diu ndm trong gidi ban cho phdp ciia Tinh sd luong vi khuan trong 1 gam theo cdng tidu chuan Viet Nam vd thi gidi. thiic: A = X. 1/K.V Kit qua phdn tich Anova vd kilm dinh LSD d 3 md hinh trdn cho thay rdng sd lupng vi khuan hieu Trong dd: A: li sd khuan lac vi khuan mgc tren khi tdng sd d md hinh 2 cap va 3 cap thap hon so vdi mgt gam. Dan vi tinh khuan lac (cfu)/gani; X: Sd md hinh 1 cap vdi mirc y nghia a = 0,05; cdn giira md khuan lac tiung bmh mgc tien dia mdi trudng khi hinh 2 cap vd 3 cap khdng cd sir sai khac cd y nghia cdy a mdt he sd pha loing; V: tiie tich (ml) mau da vl mdt thdng kl vdi mirc a = 0,05. dimg ddca'y; K: hi sdpha loang. NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011 fl
4. KHOA HOC CbNG NGHfi Bang 2. Kit qud khao sdt miic dp nhilm vi khudn hiiu khi tdng sc Max Tmng Md hinh Khdi lupng Gidi nan Sdmdu Sd mdu cd SD nuoi tdm (g) eho phep khdng dat- sd Ulu- (cfu/g) binh 1 cap 19 ±2.36 15 2,85.10^ 1,2.10^ 0.7310^ E300 R^ = 0.8188 •2250 • Y • Y °200 • ^^* —linear (Y) Lriear(Y) •5150 •••• iioo 50 0 1- 1 -+ 1 1 0 50 100 150 200 250 400 500 E.CO// Hinh 2. Mdi tuong quan giiia Fecal coliform \^ E.coli 78 NONG NGHI|P VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011
5. KHOA HOC CONG NGH£ 3. Kit qua khdo sdt mirc dp nhilm Vibrio Bang 4. Kit qud khdo sdt Vibrio trdn tdm thuang phdm Md hinh Chi tidu. gioi han Sd mau Tyle Max 1 Tmng binh SD nudi cho phdp cd sd li|u nhilm (%) '102 *102 1 cap Vibrio spp 12 80 5460 2,52 2,07 2 cdp (Chua cd quy 10 66,67 1820 0,69 0,63 3 cap dinh cu thd*) 10 66,67 980 0,37 0,33 1 cap 0 0 Vibrio choreala ''cap 0 0 1 0 3 cap 0 0 i i cap Vibrio 0 0 i o -• parahaemolyticus 0 0 ! i '- -ap 1 3 cap 0 0 0 1 ! Kit qua phan tich cho thay, Vibno spp dupc phat nghia vl mat thdng kd (a=0,05). hien tha'y trong ca 3 md hinh nudi vd tan suat xuat 4. Kit qua khao sdt muc dp nhilm Salmonella hien tuong duong nhau, d md hinh 1 cap Id 80% vd Staphyloccx:cus (12/15 mau), md hinh 2 cap va 3 cap la 66,67% Kit qua (bang 5) cho thay, d 100% cae mau tdm (10/15). Tuy nhidn, d md hinh 1 cap, mat dp te bao vi thu dupc d ca 3 md hinh nudi deu khdng cd mdt vi khuan Vibrio spp dao ddng trong khoang 1,82.10^ khuan Salmonella. Theo nghidn ciru Biii Quang Tl den 5,46.10^ cfu/g. O md hinh 2 cap va 3 cap mat dp ciing cdc cdng sir ndm 2006 [3] thi ty le mdu biin, te bao Vibrio spp lan luat d mirc 0 den 0,69.102 ^fu/g nudc ao nudi nhidm Salmonella tuong img vdi ty le va 0 den 0,37.10^ cfu/g. Theo phan tich Anova va miu tdm, ca nudi nhidm Sahnonella. Vi vdy, neu kiem dinh LSD cua 3 md hinh thi thay rdng md hinh quan ly chat lupng mdi trudng ao nudi tdt de trdnh d 1 cap khac so vdi md hinh 2 cap, 3 cap. Nhung giira 2 nhidm sinh hpc thi hodn todn cd thi cd nguydn lidu md hinh 2 cap va 3 cdp lai khdng cd sir sai khde cd y thuy san nudi khdng nhidm Salmonella. Bang 5. Kit qua khdo sdt Salmonella vd Staphylococcus Md hinh Chi tidu, gidi ban cho Sd mau Sd mdu cd Max Tmng binh SD nudi phep khdng dat sd hdu *102 *102 1 cap 0 0 2 cap Salmonella 0 0 3 cap 0 0 0 leap Staphylococcus 0 5 98 0,28.102 0,43.102 2 cap aureus 0 3 70 0,12.102 0,25.102 n=5,c=2,m=102 cfu/g, 3 cap 0 2 90 0,1.102 0,27.102 M=Wcfn/g (Trong dd: n: sdmiu kiem tia; m: muc gidi han mi tit ci cac ket qui thap han dugc coi la dat yeu ciu; M: Gidi ban cd tinh chit chip nhan, chi cin mdt ket qui vugt qui li khdng dat yeu ciu; c: Sdmiu kiem tia cd sd lugng vi khuan nim giua mviM) Theo tidu chuan chat lupng ciia cde thi tmdng nudi 1 cap, 2 cap vd 3 cap lan lupt la 0,28.102 cfu/g, Idn till gidi nhu EU, Phdp, Han Qudc, va d Viet 0,12.102 ^f^^/g ^^ o,1.102 cfu/g. Phdn tich Anova vd Nam thi chi sd nay phai bdng 0 trong 25 g thuc pham kiem dinh LSD cho thdy mat dp tl bao phdn tich. Staphylococcus spp d cde md hinh nay khdng cd su Mat dp tl bdo Staphylococcus spp dao ddng tii 0 sai khde cd y nghia vl mdt thdng kl vdi a = 0,05. - 98 cfu/g. Ty le nhilm Staphylococcus spp d tdm NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011 79
6. KHOA HQC C6NG NGH£ 5. Kit qua kilm nghi|m du lirpng hod ch^t, thudc khdng sinh Bang 6. Kit qua phdn tich tdm sii thirong phdm TT Chi tidu A3HP-1 A3HP-2 B6HP-1 B6HP-2 B8HP-1 B8HP-2 B7HP B9HP 1 Cloramphenicol KPH KPH KPH KPH KPH KPH KPH KPH 2 Nitrofuran KPH KPH KPH KPH KPH KPH KPH KPH 3 Tetracyclin KPH KPH KPH KPH KPH KPH KPH KPH 4 Sulphonamit KPH KPH KPH KPH KPH KPH KPH KPH 5 Flofenicol KPH KPH KPH KPH KPH KPH KPH KPH 6 Fluoroquinolon KPH KPH KPH KPH KPH KPH KPH KPH 7 Malachite green KPH KPH KPH KPH KPH KPH KPH KPH 8 Dipterex KPH KPH KPH KPH KPH KPH KPH KPH 9 Aflatoxin KPH KPH KPH KPH KPH KPH KPH KPH Ghi chu: KPH- Khdng phat hien Kit qua phdn tich 8 mau tdm thuong pham cho ndng tiidn"[l] vd Quyit dinh sd: 46/2007/QD-BYT thay, cdc chi tidu vl khdng sinh, hda chat vd ddc td "Quy dinh gidi han tdi da d nhilm sinh hpc vd hda nam deu khdng phdt hidn dupc trong tdm thuang hpc trong thuc pham"[2] thi tdm thuong pham thu tir pham (bang 6). cdc md hinh 3 cap deu dat ydu eau vl an todn ve sinh 6. Miic dd dam bao an todn v| sinh thuc phdm thuc pham (ATVSTP) ddi vdi nhung chi tieu (bang ciia tdm 7): Salmonella spp. Vibrio parahaemolyticus, Vibrio Theo tidu chuan cua Vi|t Nam: Thdng tu sd chorela, E.coli, Fecal coliform vi Staphylococcus 29/2010/TT-BNN&PTNT "Danh muc ehi tiiu, mirc aureus, dpe td nam Aflatoxin tdng; du lupng khdng gidi ban eho phep vl an todn we sinh thuc pham ddi sinh vd cdc thudc cam sir dung: Chloramphenicol, vdi mdt sd san pham thuc pham cd ngudn gde ddng nhdm nitrofurans. xanh malachite. vdt nhdp khau, san xuat luu thdng trong nudc thudc dipterex/Trichlorfon, nhdm tetracycline, nhdm pham vi quan ly ciia Bp Ndng nghiep vd Phdt triln sulfonamide, florfenicol, nhdm fluoroquinolones. Bang 7. Kit qua phdn tich tiiu ATVSTP tdm nudi theo 3 md hinh tai Hai Phdng, 2009 TF Chi tiiu Donv} Chi tieu tinh Cdn dat VN[3] Kit qud 1 Tdng sd VK hiiu khi CFU/g 5,0. 10^ 10« 0,3-1,2.10^ 2 Stapbilococcus aureus MPN/g <102 102-103 0,1-0.3.102 3 Fecal coliform MPN/g <W w 1.7-3,6.102 4 E. coli MPN/g 102 <2,3.102 0.8-1.5.102 5 Salmonella CFU/g 0 0 KPH 6 Vibrio cholerae CFU/g 0 0 KPH 7 Aflatoxin tdng ppb 10 15 KPH 8 Chloramphenicol ppb 0 0 KPH 9 Nhdm nitrofurans ppb 0 0 lO'H 10 Xanh malachit ppb 0 0 KPH 11 Dipterex/Trichlorfon ppb 0 0 KPH 12 Nhdm tetracyclin ppb 100 100 KPH 13 Nhdm Suhonamit ppb 100 100 KPH 14 Florfenicol ppb 1000 1000 KPH 15 Nhdm fluoroquinolones ppb 100 200 KPH Ghi cbii: KPH- khdng phit bien 80 NONG NGHIf P VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011
7. KHOA HQC CQNG NGH£ IV. KET LUAN TAI UPJ THAM KHAO 1. Cdc loai vi khuan hiiu khi tdng sd. Fecal 1. Bd NN&PTNT, 2010. Danh muc chi tieu, miic coliform, E.coh, Vibrio spp. Staphylococcus spp phdt gi&i ban cho phep ve an toan ve sinh thuc pham ddi hien dupc d ca ba md hinh 1 cap. 2 cap, 3 cap. Theo vdi mdt sd sin pham thuc pham cd ngudn gdc ddng tidu chuan tren thi gidi vd Viet Nam thi deu trong vat nhip khau, sin xui't luu thdng trong nudc thudc pham vi cho phep ve ATVSTP. Rieng Salmonella thi pham vi quan ly ciia Bd Ndng nghiep vi Phat trien chua gdp d tat ca 3 md hinh nghidn ciru. ndng tiidn. Thdng tir sd 29/2010/Tr-BNNPTNT ngay 3. Mat dp tl bdo eua vi khuan hiiu khi tdng sd, 06 thdng 5 ndm 2010 eua Bd tmdng Bd Ndng nghiep Fecal coliform. Vibrio spp d md hinh 1 cap Idn nhat va Phdt trien ndng thdn. vd cd sir sai khde cd y nghia thdng kd vdi md hinh 2 2. Bd Y tl, 2007. Quy dinh gi&i han tdi da d cap vd 3 cap; cdn giira md hinh 2 cap vd 3 cap khdng nhidm sinh hgc vi hda hgc trong there pham. Quyit cd sir sai khde ed y nghia thdng kd vdi a = 0.05. Mat dinh sd: 46/2007/QD-BYr ngdy 19 tiidng 12 ndm dp te bdo E.coli, Staphylococcus spp d md hinh 1 cap 2007 eiia Bd tiirdng Bd Y tl. Idn nhat, tuy nhien phdn tich thdng kd cho thay 3. Bili Quang Tl vd CTV, 2006. Nghien cuu giai khdng cd sir sai khde giira 3 md hinh. phip ky thuit nudi tdm sii, ca ba sa vi ca tra dim bio an toin ve sinh thuc pham. Bao cdo kit qua nghidn 4. Tdm nudi theo 1 cap, 2 cap vd 3 cap diu cuu dl tdi cap Nhd nudc. ndm 2003-2005, md sd KC- khdng chiia du lupng cloramphenicol, nitrofuran, 06-20.NN. tetracyclin. sulphonamid, flofenicol. fluoroquinolone, 4. Bd Ndng nghiip vd PTNT. 2008. Quye't dinh malachite green, dipterex, aflatoxin. Vdi ede bi|n 2670/BNN-QLCL Cdng bd danh muc cac chi tieu phdp ky thuat dd thuc hiin trong nudi thdm canh chi dinh kiem tia ddi vdi Id hing thdy san. theo 1, 2 vd 3 cap tai Hai Phdng thi san pham nudi 5. Ministry of Health, Labour and Welfare, Japan, dam bao an todn v| sinh thuc pham theo tiiu chuan 2000. Statistics of Food Poisoning Japan in 2000. Viet Nam vd qudc tl hiin hdnh. INITIAL REVIEW OF FOOD HYGIENIC AND LEVEL IN THE BLACK TIGER (Penaeus monodon) INTENSIVE SYSTEM MULTI-CYCLE AND MULTI-POND IN HAI PHONG Le Ngoc Quan, Bui Quang Te, Nguyen Thi Bien Thuy, Tmong Thi Thdnh Vinh, Hoang Thi Yen Summary Tiger prawns to ensure food safety and hygiene is very necessary, in which the solution right from farming is important. Research indicators microorganisms in black tiger shrimp (Penaeus monodon) in the form of intensive multi-level were conducted. Using quantitative methods (MNP method, S. D. Millar, and G. N. Frerchs). identifying the specific culture medium, try some of the biochemical results showed that total aerobic bacteria. Fecal Coliform, F. coli. Vibrio spp: Staphylococcus spp. Salmonella are within the permissible limits of the standards of Vietnam and world or if it does not significantly influence consumers to use products with the bacteria. However, the presence of them on the cultured species can cause disease in prawns if environmental factors such as the pond is not favorable; density of total aerobic bacteria, Fecal coliform, and Vibrio spp at level of model 1 and has the largest difference is statistically significant with model 2 and 3 levels also between model 2 and 3 grade-level differences are not statistically significant with a = 0.05; density of F. coli. Staphylococcus spp in model for the largest one, but statistical analysis showed no differences between the three models. Analyzing pesticide residues, antibiotics after the shrimp reach commercial size showed that the samples did not detect infection chloramphenicol, nitrofurans, tetracycline, sulphonamid, flofenicol, fluoroquinolone, malachite green, dipterex, aflatoxin. Keywords: Food Safety, permits, limits, standards and other errors. Ngudi phdn bi|n: TS. Hd Ky NONG NGHIf P VA PHAT TRIEN NONG THON - KY 2 - THANG 5/2011 81